Process for the isolation and purification of taxol and taxanes

Details Process for the isolation and purification of taxol and taxanes Process for the isolation and purification of taxol and taxanes

1994-08-26

1995-12-26

Marx, Irene

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing heterocyclic carbon compound having only o, n, s,...

4352404, 43524046, 43524048, 549510, 549511, C12P 1702, C12N 504

Patent

active

054787360

ABSTRACT:
A process for the separation of taxanes, particularly taxol, cephalomannine, baccatin III and deacetylbaccatin III, from ornamental yew tissue is described. The process involves a specific solvent mixture of water and 50 to 95 percent by volume methanol, ethanol or acetone and treatment of the resulting extract using activated carbon, preferably charcoal. The taxanes are preferably separated from the crude extract by a normal phase chromatographic step which preferably is through vacuum and then medium pressure column chromatographic separation, using inexpensive silica gel as an absorbent. The silica gel is regenerated by heating in a furnace above about 500.degree. C. to remove adsorbed organic materials.

REFERENCES:
patent: 5019504 (1991-05-01), Christen et al.
Gonzalez et al. (1990) Phytochemistry, 29(1), 321-22.
Pavia et al. Organic Laboratory Techniques, CBS College Publishing 1982, 488-89.
Witherup, S., et al., Journal of Natural Products 53, 1249-1255 (1990).

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