Process for the isolation and purification of taxol and taxanes

Details Process for the isolation and purification of taxol and taxanes Process for the isolation and purification of taxol and taxanes

1992-12-07

1994-01-18

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing heterocyclic carbon compound having only o, n, s,...

4352404, 43524046, 43524048, 549510, 549511, C12P 1702, C12N 500, C12N 502, C07D30500

Patent

active

052799492

ABSTRACT:
A process for the separation of taxanes, particularly taxol, from ornamental yew tissue is described. The process involves a specific solvent mixture of ethanol and water which is between 50 to 80 percent by volume ethanol and decolorizing of the resulting extract using activated carbon containing the crude taxanes. The taxanes are separated from the crude extract by a normal phase chromatographic step which preferably is through vacuum or medium pressure column chromatographic separation, using inexpensive silica gel as an absorbent.

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