Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1995-02-24
1997-10-21
Richter, Johann
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
C07D31136, C07D31140
Patent
active
056798067
ABSTRACT:
The present invention relates to a process for the isolation and purification of isoflavones from a number of different biomass sources. More particularly, the present invention relates to a three-step process whereby a biomass containing isoflavones with a solvent thereby forming an extract that is subsequently fractionated using a reverse phase matrix in combination with a step gradient elution, wherein the resulting fractions eluted from the column contain specific isoflavones that are later crystallized. The purified isoflavone glycosides may then be hydrolyzed to their respective aglycone.
REFERENCES:
"Mechanism of Antioxidant Action of Pueraria Glycoside (PG)-1 (an Isoflavonoid) and Mangiferin (a Xanthonoid)," Takashi Sato et al., Chem. Pham. Bull., 40(3):721-724, month not available (1992).
"Isolation and High Performance Liquid Chromatography (HPLC) of Isoflavonoids from the Pueraria Root," Yukio Ohshima et al., Planta medica pp. 250-254 month not available (1988).
"Induced Isoflavonoids from Copper Chloride-Treated Stems of Pueraria lobata,"Takashi Hakamatsuka et al., Phytochemistry, 30(5):1481-1482 month not available (1991).
"Isolation of New Isoflavone from Chinese Pueraria Flowers," Michinori Kubo et al., Chem. Pharm. Bull., 23(10:2449-2450, month not available (1975).
"Studies on the Constituents of Pueraria lobata V. A Trypotphan Derivative from Pueraria Flos," Jun-ei Kinjo et al., Chem. Pharm. Bull., 36(10):4171-4173, month not available (1988).
"Isoflavone Synthase from Cell Suspension Cultures of Pueraria lobata," Takashi Hakamatsuka et al., Chem. Pharm. Bull., 38(7):1942-1945, month not available (1990).
"Kudzu in the South: An old Alternative Crop," Hortscience, 26:498, month not available (1991).
"Herbal Tonic Cuts Hamsters' Alcohol Use," Science News, 144:319, (Nov. 13, 1993).
"Hamsters Take The Pledge After Ancient Chinese Cure," New Scientist, pp. 4-5, (Nov. 6, 1993).
"Isoflavone Gluosides Exist as Their 6"-0-Malonyl Esters in Pueraria lobata and its Cell Suspension Cultures," Hyung-Hwan Park et al., Chem. Pharm. Bull., 40(7) month not available (1978, 1980, 1992).
"A New Irisolidone-7-0-Glucoside and Tectoridin From Pueraria Species," Michinori Kubo and Keizo Fujita, Phytochemical Reports, 12:2547-2548, (1973).
"Isoflavonoids and the Other Constituents in Callus Tissues of Pueraria lobata," Koichi Takeya and Hideji Itokawa, Chem. Pharm. Bull., 30:1496-1499, month not available (1982).
"High-Performance Liquid Chromatographic System For A Wide Range of Naturally Occurring Glycosides," Hitomi Kaizuka and Kunio Takahashi, Journal of Chromatography, 258:135-146, month not available (1983).
"Studies on the Constituents of Pueraria lobata, III. Isoflavonoids and Related Compounds in the Roots and the Voluble Stems," Jun-ei Kinjo et al., Chem. Pharm. Bull., 35(12):4846-4850, month not available (1987).
"Genistin and its Aglucone, Genistein," E.D. Walter, J. Amer. Chem. Soc. 63:3273-3276, month not available (1941).
"Soybean Chemistry & Technology," Goss et al., 31 month not available (1944).
"Studies on the Constituents of Japanese and Chinese Crude Drugs. IV. On the Constituents of Pueraria Root. (2).," Yakugaku Zasshi, 8:688-691, month not available (1960).
"Studies on the Constituents of Pueraria lobata. IV. .sup.1) --Chemical Constituents in the Flowers and the Leaves," Kinjo et al., Chem. Pharm Bull., 36(3):1174-1179, month not available (1988).
"Oleanene Sapogenols From Puerariae Radix," Jun-ei Kinjo et al., Chem. Pharm. Bull., 33(3):1293-1297, month not available (1985).
"Kudzu (Pueraria lobata) In New England," Bruce A. Sorrie and William D. Perkins, Rhodora, 90(863):340-343, (1988).
"Volatile Flavor Components of Puerariae Radix (Pueraria lobata Ohwi)," Mistuo Miyazawa and Hiromu Kameoka, Agric. Bio. Chem., 52(4):1053-1055, month not available (1988).
"Fruits and Vegetable Fight Some Cancers", Better Nutrition for Today's Living, 55(10):26, (Oct. 1993).
"Spicing up your Isoflavones and Phytosterols," University of California, Berkeley Wellness Letter, 10(1):1, (Oct. 1993).
"Can Tofu Stop Cancer?", Susan Brink, U.S. News & World Report, 114(21):77, (May 31, 1993).
"Genistein, a Dietary Derived Inhibitor of In Vitro Angiogenesis," Cancer Researcher Weekly, p. 23, (May 17, 1993).
"Red Clover: Herbal Healer," Rob McCaleb, Better Nutrition for Today's Living, 53(12):28, (Dec. 1991).
"Increasing Use of Soyfoods and their Potential Role in Cancer Prevention," Mark Messina and Virginia Messida, Journal of the American Dietetic Association, 91(7):836, (Jul. 1991).
"Is This the Dawning of the Age of Soy?", Chilton's, Food Engineering, 65(10):32, (Oct. 1993).
"Meals that Heal. Fruit Salad that Builds Bones. Soup that Lowers Blood Pressure. The Nutraceutical Revolution is Here|", Carl Sherman, Health, 23(2):69, (Mar. 1991).
"Blocking Breast Cancer: Do Faulty Estrogen Receptors Make a Meaner, Tougher Tumor?", Kathy A. Fackelmann, Science News, 137(19):296, (1990).
"Separation of Soybean Isoflavones from Their 5-Hydroxy Derivatives by Thin-Layer Chromatography," L.C. Wang, Short Communications, pp. 296-298 month not available (1971).
"Isoflavonoid Constituents of Soybeans and Isolation of a New Acetyl Daidzin," Ohta et al., Agri Biol. Chem., 43(7):1415-1419,(1979).
"Removal of Phenolic Compounds from Soy Protein Extracts Using Activated Carbon," J.S.L. How and C.V. Morr, Journal of Food Science, 47:933-940, month not available (1982).
"Determination of Isoflavones in Soybean Flours, Protein Concentrates, and Isolates," Arthur C. Eldridge, J. Agric. Food Chem., 30(2):353-355, month not available (1982).
"Isolation of 6"-0-Acetylgenistin and 6"-0-Acetyldaidzin from Toasted Defatted Soyflakes," Efi Farmakalidis and Patricia A. Murphy, J. Agric. Food Chem., 33(3):385-389, month not available (1985).
"High-Performance Liquid Chromatographic Analysis of Phytoestrogens in Soy Protein Preparations with Ultraviolet, Electrochemical and Thermospray Mass Spectrometric Detection," K.D.R. Setchell and Mary Beth Welsh, Journal of Chromatography, 386:315-323, month not available (1987).
"Identification and Accumulation of Isoflavonoids and Isoflavone Glucosides in Soybean Leaves and Hypocotyls in Resistance Responses to Phytophthora megasperma f.sp. glycinea," Morris et at., Physiological and Molecular Plant Pathology 39:229-244, month not available (1991).
"Flavonoids of Various Prunus Species VI. The Flavonoids in the Wood of Prunus aequinoctialis, P. nipponica, P. maximow and P. avium," Masao Hasegawa, J. Am. Chem. Soc., 79:1738-1740, month not available (1957).
"Chemical Determination of Isoflavones," Edmund Cheng and Lester Yoder, Iowa Academy of Science, 65:220-223, month not available (1958).
"The Constituents of Pueraria Root," Chem. Pharm. Bull., 7:134-136, month not available (1959).
"Chemosystematics of the Leguminosae. Flavonoid and Isoflavonoid Patterns in the Tribe Genisteae," J.B. Harborne, Phytocemistry, 8:1449-1456, month not available (1969).
"Distribution of Flavonoids in the Genus Baptisia (Leguminosae)," Markham et al., Phytochemistry, 9:2359-2364, month not available (1970).
"14.2 Isoflavones," Harborne et al., The Flavonoids, pp. 746-757, month not available (1975).
"A New Isoflavone from Soya Beans," Naim et al., Phytochemistry, 12:169-170, month not available (1973).
"Chemical Studies on the Oriental Plant Drugs. XXXV. The Chemical Constituents of the Heartwood of Maackia amurensis var. buergeri," Takai et al., Chem. Pharm. Bull., 20(11):2488-2490, month not available (1972).
"Soybean Isoflavones. Characterization, Determination, and Antifungal Activity," Naim et al., J. Agric. Food Chemistry, 22:806-809, month not available (1979).
".beta.-Galactosidase-Inhibiting New Isoflavonoids Produced by Actinomycetes," Hazato et at., The Journal of Antibiotics, XXXII(3):217-222, month not available (1979).
"Organoleptic and Nutritional Effects of Phenolic Compounds on Oilseed Protein Products: A Review," F. Sosulski, Journal of the American Oil Chemists' Society, 56:711-715, month not available (1979).
"Isolation of a New Isoflavone Acetyl Glucoside, 6"-0-Acetyl
Bailey David T.
Sullivan James L.
Yegge John A.
Zheng BoLin
Hauser, Inc.
Petersen Steven C.
Richter Johann
Stockton Laura L.
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