Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2007-04-17
2007-04-17
Wax, Robert A. (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S395000
Reexamination Certificate
active
10399118
ABSTRACT:
The present invention provides a process for the preparation of Avidin having a high purity of 98% from egg white, the said process comprising lyophilizing the homogenized egg white with buffer followed by equilibrating the lyophilized material with cation exchanger for an hour at a temperature range of 20° to 30° C.; filtering the mixture to obtain a filtrate and matrix residue; washing the matrix twice with buffer; separating the washings to obtain washed matrix; adding 0.01 M dye in buffer to the washed matrix; equilibrated for an hour at an ambient temperature; separating the supernatant; adding acetic acid to supernatant and dialysing the solution till it is decolorised to obtain pure Avidin.
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patent: WO 00/27814 (2000-05-01), None
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DATABASE WPI “Avidin elution—comprises absorption of basic protein on cation exchange resin”, AN 1987-040958 & JP 62 000500 A (QP CORP), abstract (1987).
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DATABASE BIOSIS ′Online! Piskarev Ve et al, “A novel preparative method for the isolation of avidin and riboflavin-binding glycoprotein from chicken egg-white by the use of high-performance liquid chromatography”, Database accession No. PREV199089125572, abstract (1990).
Gupta Munishwar Nath
Rao Mukkavilli Venkata Subba
Roy Ipsita
Drinker Biddle & Reath LLP
Indian Institute of Technology
Monaco Daniel A.
Wax Robert A.
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