Process for the isolation and characterization of a gene enzyme

Details Process for the isolation and characterization of a gene enzyme Process for the isolation and characterization of a gene enzyme

1995-05-23

1999-09-07

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 712, 435830, 536 232, 536 237, C12N 120, C12N 1531, C12N 1552, C12P 2100

Patent

active

059486708

ABSTRACT:
A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.

REFERENCES:
patent: 4810648 (1989-03-01), Stalker
Brandsch, et al. (1987) Eur. J. Biochem. 167 pp. 315-320.
C. O. Knowles, et al. "Microbial Degradation of the Carbamate Pesticides Desmedipham, Phenmedipham, Promecard, and Propamocarb", Bull. Environm. Contam. Toxicol., 27, 529-533 (1981).
David N. Radin, et al., "Herbicide-tolerant tobacco mutants selected in situ and recovered via regeneration from cell structure" Genet. Res. Camb., (1978), 32, pp. 85-89.

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