Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-05-23
1998-12-08
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
300325, 300326, 435 6, 530 2432, 530416, 530417, 530420, C07H 2104, C07K 136, C07K 708, C07K 1400
Patent
active
058468030
ABSTRACT:
A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.
REFERENCES:
patent: 4810648 (1989-03-01), Stalker
C. O. Knowles et al. "Microbial Degradation of the Carbamate Pesticides Desmedipham, Phenmedipham, Promecarb, and Propamocarb",Bull. Environm. Contam. Toxicol., 27, 529-533 (1981).
David N. Radin et al. "Herbicide-tolerant tobacco mutants selected in situ and recovered via regeneration from cell structure" Genet. Res. Camb., (1978), 32, pp. 85-89.
Boidol Werner
Pohlenz Hans-Dieter
Streber Wolfgang
Hoechst Schering AgrEvo GmbH
Nelson Amy J.
Robinson Douglas W.
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