Process for the isolation and characterisation of a gene enzyme

Details Process for the isolation and characterisation of a gene enzyme Process for the isolation and characterisation of a gene enzyme

1994-02-14

1996-08-06

Chereskin, Che S.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 232, 536 242, 536 241, 4353201, C12N 1582, C12N 1531, C12N 1555, C12N 1567

Patent

active

055433065

ABSTRACT:
A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.

REFERENCES:
patent: 4810648 (1989-03-01), Stalker
C. O. Knowles, et al. "Microbial Degradation of the Carbamate Pesticides Desmedipham, Phenmedipham, Promecarb, and Propamocarb", Bull. Environm. Contam. Toxicol., 27, 529-533 (1981).
David N. Radin, et al. "Herbicide-tolerant tobacco mutants selected in situand recovered via regeneration from cell structure"Genet. Res. Camb., (1978), 32, pp. 85-89.
Knowles, et al. Bull. Environ. Contam. Toxicol. 27: 529-533 (1981).

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