Process for the intracellular over-production of...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C424S093100, C424S093200, C424S093400, C424S241100, C424S282100, C435S041000, C435S069100, C435S070100, C435S071100, C435S216000, C435S243000, C435S252100, C435S252300, C435S252330, C435S320100

Reexamination Certificate

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10746046

ABSTRACT:
An improved process for the production of streptokinase using a genetically engineered strain ofEscherichia coliwhich overproduces streptokinase intracellularly and more particularly, the overall process disclosed herein, concerns with an improvement in the fermentative production of streptokinase using an optimized growth medium mainly comprised of simple salts and trace-elements; thus, in principal, the present process constitutes an improved and more economical means for the production of streptokinase which may be useful in thrombolytic therapy.

REFERENCES:
patent: 5187098 (1993-02-01), Malke et al.
Dao et al. 1985. Appl. Environ. Microbio. vol. 49(1): 115-119.

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