Process for the incorporation of foreign dna into the genome of

Details Process for the incorporation of foreign dna into the genome of Process for the incorporation of foreign dna into the genome of

1984-02-23

1990-07-10

Tarcza, J. E.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

4351723, 435 91, 4352522, 435320, 935 30, 935 35, 935 56, 935 64, 935 67, 800DIG9, 800DIG44, 800DIG43, 800DIG23, C12N 1500, C12N 120, C12N 100, C12P 1934

Patent

active

049408380

ABSTRACT:
The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of a Ti (tumour inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria wherein at least one plasmid which has the vir-region of a Ti (tumour inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.

REFERENCES:
patent: 4399216 (1983-08-01), Axel et al.
patent: 4459355 (1984-07-01), Cello et al.
Hoekema et al., "A Binary Plant Vector Strategy Based on Separation of vir and T-Region of the Agrobacterium Tumefaciens", Nature 303, pp. 179-180 (1983).
Klee et al., "Complementation Analysis of Agrobacterium Tumefaciens Ti Plasmid Mutations Affecting Oncogenicity", Journal of Bacteriology 150(1), pp. 327-331 (1982).
Hernalsteens et al., "The Agrobacterium Tumefaciens Ti Plasmid as a Host Vector System for Introducing Foreign DNA in Plant Cells", Nature 287, pp. 654-656 (1980).
De Greve et al., "Regeneration of Normal and Fertile Plants that Express Octapine Synthase from Tobacco Crown Galls After Deletion", Nature 300, pp. 752-755 (1982).
Yang et al., "Revertant Seedlings from Crown Gall Tumors Retain a Portion of the Bacterial Ti Plasmid DNA Sequences", Proceedings of the National Academy of Sciences 78(7), pp. 4151-4155 (1981).
Garfinkel et al., "Genetic Analysis of Crown Gall: Fine Structure Map of the T-DNA by Site-Directed Mutagenesis", Call 27XI, pp. 143-153 (1981).

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