Process for the incorporation of foreign DNA into the genome of

Details Process for the incorporation of foreign DNA into the genome of Process for the incorporation of foreign DNA into the genome of

1984-02-23

1987-09-15

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435 70, 435253, 435320, 47 58, 935 30, 935 56, 935 64, 935 67, C12P 2100, C12P 2102, C12N 122, C12N 100

Patent

active

046939768

ABSTRACT:
The invention relates to a process for the incorporation of foreign DNA into the genome of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium tumefaciens bacteria, which contain one or more Ti (tumour inducing) plasmids, wherein as Ti plasmid a stable cointegrate plasmid composed of the plasmid R772 and the plasmid pTiB6 with foreign DNA incorporated in the T-region of the Ti component of the cointegrate plasmid is applied as well as to a cointegrate plasmid pAL969 and cointegrate plasmids derived from the cointegrate plasmid by the incorporation of foreign DNA into the T-region of the Ti component.

REFERENCES:
Hooykaas et al, Plasmid vol. 4, pp. 64-75, "Molecular Mechanism of Ti Plasmid Mobilization by R Plasmids: Isolation of Ti Plasmids with Transposer Insertions in Agrobacterium-Tumetusciens.
Hille et al, Abstract P-40 Symposium University Bielefeld Autumn 1982, "Onc and Vir Genes Located on the Ti-Plasmid of Agrobacterium Tumefaciens".
Leemans et al, J Mol Appl Genet, vol. 1(2), 1981, "Site-Specific Mutagenesis of Agrobacterium Ti Plasmids and Transfer of Genes to Plant Cells".
Schell et al in Genetic Eng to Biotech 1982 "The Use of Ti Plasmids as Gene Vectors for Plants" pp. 41-53.
Hille et al Plasmid vol. 7, pp. 107-118, 1982 "Construction and Application of R Prime Plasmids . . . for Complementation of vir Genes".
Ruvkun et al, Nature, vol. 289, pp. 85-88, 1981.
White et al, Proc Natl Acad Sci, vol. 79, May 1982, pp. 3193-3197, "Tumor Induction by . . . to the Plant Genome".
Molecular Genetics of the Bacteria-Plant Interaction, pp. 223-228, Symposium Plasmid vol. 7 pp. 107-118 (1982) J. Hille et al.
Journal of Bacteriology, vol. 154, May 1983 J. Hille et al.

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