Process for the in-vitro transformation of plant protoplasts wit

Details Process for the in-vitro transformation of plant protoplasts wit Process for the in-vitro transformation of plant protoplasts wit

1985-07-29

1987-08-04

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43524047, 935 56, 800 1, C12N 1500, C12N 500

Patent

active

046846115

ABSTRACT:
Genetic properties of higher plants are transformed by incubating protoplasts of the plants together with plasmid DNA. The incubation is effected in the presence of polyethylene glycol and calcium ions. Subsequently--during after-incubation--the calcium ion concentration in the incubation medium is gradually increased and at the same time the polyethylene glycol concentration is decreased. The resulting aggregates are separated, grown further separately, and examined for modification of their genetic properties.

REFERENCES:
patent: 4394448 (1983-07-01), Szoka, Jr. et al.
Lurquin, Archives Internationales de Physiologie et de Biochemie, vol. 87(4), pp. 824-825 (1979).
Lurquin, Plant Science Letters, vol. 21, pp. 31-40 (1981).
Davey et al., Plant Science Letters, vol. 18, pp. 307-313 (1980).
Kado, Recombinant DNA Technical Bulletin of the NiH, vol. 2, pp. 145-153 (1980).
Otten et al., Molec. Gen. Genet., vol. 183, pp. 209-213, (1981).
Fraley et al., Current Topics in Microbiology and Immunology, vol. 96, pp. 171-191 (1982).

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