Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1985-05-22
1992-04-21
Nucker, Christine
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43524031, 43524021, 424 88, C12N 502, A61K 3900
Patent
active
051067463
ABSTRACT:
A process for the in vitro immunization of an immuno-competent splenocyte against an immunogen comprising (a) obtaining a first human splenocyte population, (b) fractionating the first population so that the first fraction is enriched with T-cells and a second fraction is enriched with B-cells, (c) mixing together the cells from the first and second fractions to form a second population having a T-helper cell to B-cell ratio of at least 0.4, and the percentage of T-suppressor cells is essentially unchanged from the first population, and (d) culturing the second population in a medium containing human serum, an immunogen and a lymphokine or lymphokines which induce proliferation and differentiation of T and B cells.
REFERENCES:
patent: 4668629 (1987-05-01), Kaplan
Dosch, H. and Gelfand, E. W., Journal of Immunology, vol. 118, No. 1, pp. 302-308, 1977.
Mishell & Dutton, "Immunization of Dissociated Spleen Cell Cultures from Normal Mice", J. Exp. Med. 126:423 (1967).
Borrebaeck et al., Chem. Abs. 44110q, vol. 102, No. 5, p. 425, Feb. 4, 1985.
Borrebaeck, Acta Chemica Scandinavica, pp. 647-648, vol. B37, No. 7 (1983).
Ho & Durda, J. Cell. Bio., Supp. No. 9, Part A, p. 41, No. 0090 (1985).
Cunningham T.
E. I. Du Pont de Nemours and Company
Nucker Christine
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