Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2008-01-08
2008-01-08
Graser, Jennifer E. (Department: 1645)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C530S388100, C424S130100, C424S141100, C424S151100, C435S346000, C435S326000
Reexamination Certificate
active
10260497
ABSTRACT:
The process of the invention comprises the implementation of axenic conditions, with use of a liquid single-phase culture medium. For obtaining the amastigote forms, this medium is buffered at a pH of 5.5 to 6.5 and has an osmolarity of at least 400 milliosmoles/kg of liquid, and in particular 400 to 550 milliosmoles/kg of liquid. For obtaining promastigote forms, this medium is buffered at a pH of 7 to 7.5 and has an osmolarity of at least 300 milliosmoles/kg of liquid. This process allows the adaptation and culture in vitro of different stages of tissular parasites, such as leishmanias andT. cruzior also hematoprotozoa.
REFERENCES:
patent: 3911097 (1975-10-01), Hanson
patent: 3993743 (1976-11-01), Hanson
patent: 4687666 (1987-08-01), O'Daly
patent: 0 420 635 (1991-04-01), None
patent: 1 518 449 (1968-03-01), None
patent: 83/01785 (1983-05-01), None
patent: 86/02098 (1986-04-01), None
patent: 93/03167 (1993-02-01), None
Jadin et al, “Acquisitions recentes dans les techniques de culture desTrypanosomes africains”, Annales Des Societes Belges De Medecine Tropicale 49(4):331-340 (1969).
Lemesre et al, “Requirements of defined cultivation conditions for standard growth ofLeishmania promastigotesin vitro”, Acta Tropica 45(2):99-108 (1988).
Joshi et al, “Cloning and characterization of differentially expressed genes from in vitro-grown amastigotes' ofLeishmania donovani”, Molecular and Biochemical Parasitology 58(2):345-354 (1993).
Hart et al, “Transformation in vitro ofLeishmania mexicanaamastigotes to promastigotes: nutritional requirements and the effect of drugs”, Parasitology 83:529-541 (1981).
Kweider et al, “Development of metacicLeismania pastiesis associated ith the increasing expression GP65.e major surface antigen”, Parasite Immunol 11:197-209 (1989).
O'Daly et al, “Differential growth requirements of severalLeishmaniaspp. in chemically defined culture media”, Acta Tropica 45:109-126 (1988).
De Castro et al, “Trypansoma cruzi: Adrenergic Modulation of Cyclic AMP Role in Proliferation and Differentiation of Amastigotes in Vitro”, Experimental Parasitology 64:368-375 (1987).
Velasco et al, “A Survey of Culture Media ofTrycanosoma cruziAmastigote Forms From Infected Vero Cells”, Chemosphere 19(10/11):1743-1748 (1989).
Velasco et al, “In Vitro Survival of Amastigote Forms ofTrypanosoma cruziin Media Conditioned By Vero Cells”, Chemosphere 21(1-2):263-268 (1990).
Bates et al. Parasitology 105(2):193-202 (1992).
Bates, P.A. ParasitologyToday 9(4):143-146 (1993).
Chaulhuri et al. Indian. J. Med. Res. 84:457-460 (1986).
Childs et al. Int. J. Parasitology 8(4):255-258 (1978).
Hirumi et al. Parasitology 102(2):225-236 (1991).
Hirumi et al. J. Parasitology 75(6):985-989.
Lima et al. J. Protozool. 35(1):108-110 (1988).
Graser Jennifer E.
Institut Francais de Recherche Scientifique pour le Developpemen
Nixon & Vanderhye P.C.
LandOfFree
Process for the in vitro culture of different stages of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for the in vitro culture of different stages of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the in vitro culture of different stages of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3916072