Process for the immobilization of cyclodextrine glycosyltransfer

Details Process for the immobilization of cyclodextrine glycosyltransfer Process for the immobilization of cyclodextrine glycosyltransfer

1983-08-24

1986-06-03

Warden, Robert J.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435177, 435178, 435179, 435180, C12N 1106, C12N 1102, C12N 1110, C12N 1108

Patent

active

045930040

ABSTRACT:
The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme.
The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained.
The advantage of the process of the present invention is that the thus immobilized enzyme--contrary to the dissolved enzyme--is suitable for longlasting application and can be readily used on industrial scale continuous production.

REFERENCES:
patent: 4338398 (1982-07-01), Yoneyama
patent: 4525457 (1985-06-01), Sakata et al.
Zaborsky, O. Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973 (pp. 26-27).
Biotechnol. Bioeng. 19, pp. 87-99, Nakamura et al. (1977).

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