Process for the immobilization of compounds comprising nucleophi

Details Process for the immobilization of compounds comprising nucleophi Process for the immobilization of compounds comprising nucleophi

1983-08-24

1987-06-02

Marantz, Sidney

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435174, 435177, 435181, 436532, 525 541, 525 542, 525384, C12N 1108, C12N 1102, C12N 1106, C12N 1100

Patent

active

046703900

ABSTRACT:
The invention relates to a process for the immobilization of compounds comprising nucleophilic groups.
According to the process of the invention a polymer comprising an amido group is swollen in a buffer. For this purpose any buffer is suitable which contains no amino, sulfhydryl and/or hydroxy groups in the molecule. To the swollen polymer a solution of a quinone - preferably p-benzoquinone - in a water miscible organic solvent is added and the mixture is activated at 273.degree.-343.degree. K. for 0.5-48 hours - preferably for 24 hours. The unreacted quinone is washed out with an aqueous solution of a water miscible organic solvent and thereafter water and/or a buffer. To the purified gel at a pH value between 3 and 11 - preferably between 6 and 8 - a buffered solution of a compound comprising a nucleophilic group is added, the suspension is incubated at 260.degree.-313.degree. K. for 24 hours, the gel is separated and the non-bound compound comprising a nucleophilic group is removed by washing.

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