Process for the genetic modification of yeast

Details Process for the genetic modification of yeast Process for the genetic modification of yeast

1987-06-26

1990-06-26

Teskin, Robin

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435320, 435255, 435257, 435 696, 935 28, 935 56, 935 69, C12P 2100, C12P 2102, C12N 1500

Patent

active

049371932

ABSTRACT:
Yeast is genetically modified by transformation with an integration vector comprising two copies of a homologous 2 .mu.m plasmid DNA sequence in direct orientation relative to one another and encompassing the said DNA sequence, and then isolating, from the transformed yeast obtained, cells containing the endogenous 2 .mu.m plasmid modified by incorporation of the said DNA sequence but not containing the said vector. The resulting yeast can be maintained under non-selective growth conditions.

REFERENCES:
patent: 4775622 (1988-10-01), Hitzeman et al.
Constructing of a Yeast Plasmid Cloning Vector with High Stability in Saccharomyces Strains Deficient in 2-.mu.m.--GB 2 175 590.
Cloning with 2-.mu.m DNA Vectors and the Expression of Foreign Genes in Saccharomyces Bachmair & Ruis (1984) Monatshefte fur Chemie 115, 1229-11235.
Jayaram, M., Association of Reciprocal Exchange with Gene Conversion Between the Repeated Segments of 2-.mu.m Circle; J. Mol. Biol. 191, p. 341 (1986).
Henderson, R. C. A.; The Transformation of Brewing Yeasts with a Plasmid Containing the Gene for Copper Resistance; Current Genetics 9, pp. 133-138 (1985).
Jayaram, M.; Mating Type-Like Conversion Promoted by 2 micron specific recombinase; Molec. Cell. Biol. 6 (11), pp. 3831-3837 (1986).
Struhl, K.; Direct Selection for Gene Replacement Events in Yeast; Gene 26, pp. 231-242 (1983).

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