Process for the extraction, purification and enzymatic...

Drug – bio-affecting and body treating compositions – Plant material or plant extract of undetermined constitution... – Containing or obtained from leguminosae

Reexamination Certificate

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Reexamination Certificate

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07465467

ABSTRACT:
A process for the extraction, purification and enzymatic modification of β-conglycinin α′ subunit, wherein β-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the α′ subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).

REFERENCES:
patent: 4409248 (1983-10-01), Lehnhardt et al.
patent: 6171640 (2001-01-01), Bringe
patent: 6365802 (2002-04-01), Kridl
patent: 6541259 (2003-04-01), Lassner et al.
patent: 2001/0024677 (2001-09-01), Bringe
patent: 0 797 928 (1997-10-01), None
patent: 1 172 087 (2002-01-01), None
patent: 2 784 029 (2000-04-01), None
patent: 00/77034 (2000-12-01), None
Wu et al., “Pilot-plant fractionation of soybean glycinin and beta -conglycinin.” Journal of the American Oil Chemists' Society, vol. 76, No. 3, 1999, pp. 285-293, XP002243567.
Lovati et al. “Soy protein peptides regulate cholesterol homeostasis in Hep G2 cells.” Journal of Nutrition, vol. 130, No. 10, Oct. 2000 pp. 2543-2549, XP002243172 ISSN: 0022-3166.
Lovati et al. “Soybean protein products as regulators of liver low-density lipoprotein receptors I. indentification of active betta-conglycinin subunits” Journal of Agricultural and Food Chemistry, American Chemical Society, Washington, US, vol. 46, No. 7, 1998, pp. 2474-2480, XP002928070 ISSN: 0021-8561.
Manzoni et al. “Soybean protein products as regulators of liver low-density lipoprotein receptors. II. alpha alpha su rich commercial soy concentrate and alpha su defficient mutant differently affect low-density lipoprotein receptor activation.” Journal of Agricultural and Food Chemistry, vol. 46, No. 7, 1998, pp. 2481-2484, XP002243568.
Database WPI, Section Ch, Week 199433 Derwent Publications Ltd., London, GB; AN 1994-268692 XP 002243569 & JP 06 197788 (Fuji Seiyu KK), Jul. 19, 1994.

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