Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1976-04-05
1977-05-31
Moyer, Donald B.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
424 94, A61K 3558
Patent
active
040270122
ABSTRACT:
A process for extracting and isolating from snake venoms the enzymatic component having "in vitro" coagulant activity and "in vivo" anticoagulant activity from the other components having "in vitro" coagulant activity and from the protein components, wherein the snake venom, dissolved in a medium suitable for chromatographic separation treatment is passed through a column filled with a solid support for selective absorption of said in vivo anticoagulant enzyme, not soluble in aqueous solvents, bound to a substratum or inhibitor of said enzyme, a first elution medium at pH 6-7 and molarity less than or equal to 0.05M is passed through said filling, wherein said venom has been introduced, the eluate is collected in subsequent portions until the elution of more than 95% of the protein components of the venom and an "in vitro" coagulant fraction, different from that present in the second eluate are obtained, a second elution medium at a pH even greater than 9 and molarity greater than or equal to 0.3M is passed through, the eluate is collected in subsequent portions until the elution of the in vivo anticoagulant enzyme absorbed in the column, together with less than 5% of protein components are obtained, anticoagulant pharmacological preparation obtained according to the above process.
REFERENCES:
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patent: 3711376 (1973-01-01), Hatton et al.
patent: 3743722 (1973-07-01), Nolan
patent: 3761586 (1973-09-01), Matsumoto et al.
patent: 3819605 (1974-06-01), Holleman et al.
patent: 3879369 (1975-04-01), Nolan
Brockway et al., J. of Biological Chem., vol. 246, No. 14, Issue of July 25, 1971, pp. 4641-4647.
Bjorkman, Acta. Chem. Scand., 26(1972) pp. 1111-1116.
Axen et al., Acta. Chem. Scand., 25(1971) pp. 2711-2716.
Chemical Abstracts, vol. 78 (1973) 94129b & vol. 84 (1976) 131875w.
Laboratori Biochimici Fargal-Pharmasint S.p.A.
Moyer Donald B.
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