Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
2003-11-14
2009-10-27
Tsang, Cecilia (Department: 1654)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C530S344000, C530S303000
Reexamination Certificate
active
07608583
ABSTRACT:
The present invention relates to the extraction and isolation of insulins from recombinant sources, particularly those expressed in and secreted by yeasts. Organic solvents have been used to extract host surface bound forms of insulin polypeptide. In addition, procedures for combining the steps medium clarification, solvent extraction and chromatography, in order to effect the simultaneous isolation and purification of soluble and membrane bound forms of insulin, is disclosed.
REFERENCES:
patent: 7091032 (2006-08-01), Annibali
Willis “Expanded Bed Adsorption” Modern Drug Discov., 2001, 4, 43-44.
Trinh et al. “Recovery of mouse endostatin produced byPichia pastorisusing expanded bed adsorption.” Bioseparation, 2000, 9, 223-30.
Scopes, Protein Purification: Principles and Practice, Springer, New York, 1994, pp. 157-171 and 85-93.
Gerlough & Bates “The Purification and Some Properties of Insulin,” J. Pharm. Exp. Therapeutics, 1932, vol. XLV, No. 1, pp. 19-51.
Wang et al. (“Human Insulin from a Precursor Overexpressed in the Methylotropic YeastPichia pastorisand a Simple Procedure for Purifying the Expression Product,” Biotechnology and Bioengineering, 2001, 73, 74-79).
Raju Edupuganti B
Sahib Maharaj K
Shaligram Umesh S
Bio Intellectual Property Services (Bio IPS LLC). O. M. (Sam) Za
Bradley Christina
Tsang Cecilia
Wockhardt Limited
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