Process for the determination of peroxidase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 27, 435 28, 435184, G01N 33535, C12Q 130, C12Q 128, C12N 999

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active

047525703

ABSTRACT:
The present invention provides a process for the determination of peroxidase by the addition of a peroxide and of a chromogen and measurement of the color resulting from the oxidation of the chromogen, the color formation being stopped after a definite time by the addition of a stop agent, wherein catalase is used as stop agent.

REFERENCES:
patent: 4155868 (1979-05-01), Kaplan et al.
patent: 4615972 (1986-10-01), Gallacher
Chemical Abstracts, Band 99, Nr. 5, 01, Aug. 1983, "Adaptation of the Ngo-Lenhoff Peroxidase Assay for Solid Phase ELISA".
Feinstein, "Perborate As Substrate In a New Assay of Catalase", pp. 1197-1201 (1949).
Van Vunakis, et al., Meth. Enzymol. 70: 432 (1980).
Bovaird, et al., Clin. Chem. 28(12): 2423-2426 (1982).
Rook, Lepr. Rev. 52: 281-285 (1981).
Gallati, Clin. Chem. Clin. Biochem. 17: 1-7 (1979).
Al-Kaissi, et al., J. Immunol. Meth. 58: 127-132 (1983).
Chance, B. et al, (1964) Methods in Enzymology vol. 2, pp. 764-775.
Lenhoff et al, (1964) Methods in Enzymology vol. 2, pp. 758-764.
Singh et al, (1980) Anolytica Chimica Acta, vol. 115, pp. 401-405.

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