Process for the determination of free fatty acids

Details Process for the determination of free fatty acids Process for the determination of free fatty acids

1980-12-02

1982-11-23

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 15, 435 25, 435 28, 435189, C12Q 100, C12Q 148, C12Q 128, C12Q 126

Patent

active

043605915

ABSTRACT:
This invention relates to a process for the determination of free fatty acids comprising a reaction system 1 in which acyl coenzyme A synthetase is allowed to act on the free fatty acids in the presence of adenosine triphosphate and coenzyme A to yield acyl coenzyme A and a reaction system 2 in which acyl coenzyme A oxidase is allowed to act on the acyl coenzyme A to form hydrogen peroxide, followed by the determination thereof, said process being characterized in that myokinase is added in the reaction system 1 to make it go rapidly to completion.

REFERENCES:
patent: 3886045 (1975-05-01), Meiattini
patent: 4071413 (1978-01-01), Takahashi et al.
patent: 4251629 (1981-02-01), Yumanishi et al.
patent: 4301244 (1981-11-01), Kikuchi et al.
Stokes et al., "A Soluble Acyl-Coenzyme A Oxidase from the Yeast Candida utilis", Arch. Biochem. Biophys., vol. 176, (1976), pp. 591-603.

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