Chemistry: analytical and immunological testing – Involving iga – igd – ige – or igm
Patent
1992-07-30
1994-12-27
Saunders, David
Chemistry: analytical and immunological testing
Involving iga, igd, ige, or igm
436501, 436512, 436536, 436538, 436540, 436541, 435 792, 435 793, 435 794, 435 795, G01N 33563, G01N 33566, G01N 33536, G01N 33537
Patent
active
053765575
ABSTRACT:
The present invention provides a method for type determination in body fluids of antibodies of a definite immunoglobulin class which are directed against an antigen A according to the immunoassay principle by incubation of the sample solution with at least two receptors R.sup.1 and R.sup.2, of which R.sup.1 is immobilizable and binds with the antibody to be determined and R.sup.2 is labelled and binds either with the antibody to be determined or with the antigen A, separation of the solid phase from the liquid phase and measurement of the label in one of the two phases, wherein the immunological reaction is carried out in homogeneous phase and the sample solution is incubated simultaneously with receptor R.sup.1 and an excess of human antibodies or fragments thereof which belong to the same immunoglobulin class as the antibody to be determined and are not bindable with the antigen A and subsequently receptor R.sup.2 and possibly further receptors are added thereto. The present invention also provides a reagent for carrying out this method.
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Boehringer Mannheim GmbH
Preston D. R.
Saunders David
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