Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-02-28
1990-10-30
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435176, 436501, 436513, 436517, 436518, 436524, 436528, 436537, 436540, 436819, 436824, 530810, G01N 3353, G01N 33557, G01N 33543, G01N 33542
Patent
active
049668390
ABSTRACT:
The present invention provides a process for the determination of a bindable analyte according to the principle of heterogeneous immunoassay by incubation of a sample solution which contains the analyte with a labelled first receptor specifically bindable with the analyte and present in dissolved phase and a second receptor present in a solid phase which does not cross-react with the first receptor and can fix a complex which contains the analyte and first receptor, separation of the phases after incubation and quantitative measurement of the labelling bound to the solid phase, wherein there is determined the back dissociation velocity of the labelling bound to the solid phase into the dissolved phase and the quotients of the back dissociation velocity and measurement value are used as a measure for the correctness of the test result of the first measurement.
REFERENCES:
patent: 4381291 (1983-04-01), Ekins
patent: 4459359 (1984-07-01), Neurath
patent: 4462964 (1984-07-01), Gaigan
patent: 4595661 (1986-06-01), Cragle et al.
patent: 4673653 (1987-06-01), Gaigan
patent: 4690899 (1987-09-01), Klose et al.
Boehringer Mannheim GmbH
Hoffer Florina B.
Kepplinger Esther L.
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