Process for the determination of an antibody in human body fluid

Details Process for the determination of an antibody in human body fluid Process for the determination of an antibody in human body fluid

1988-05-12

1990-05-29

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

436513, 436518, 436530, 436819, 436826, 436512, 435 7, G01N 33563, G01N 33543, G01N 33544, G01N 3353

Patent

active

049295438

ABSTRACT:
The present invention provides a process for the determination of an antibody in human body fluids according to the immunoassay principle in which a sample containing the antibody to be determined is incubated with at least two different receptors R.sub.1 and R.sub.2, of which one receptor R.sub.1 carries an antigenic determinant specific for the antibody to be determined and one receptor R.sub.2 carries a label to form bound and unbound label, the part which contains the bound label is separated from the part which contains the unbound label and the label is measured in one of the two parts, wherein, for the control, instead of the sample, there is used a standard solution which contains a conjugate of a bindable non-human antibody or a Fab or F(ab').sub.2 fragment thereof which non-human component binds with a receptor R.sub.s which also binds to the antibody to be determined, and a human immunoglobulin or the Fc part thereof. The present invention also provides a control reagent for the determination of an antibody in human body fluids according to the immunoassay principle, containing a conjugate of an antibody bindable with the receptor R.sub.s specific for the antibody to be determined or a Fab or F(ab').sub.2 fragment thereof with a human immunoglobulin or the Fc part thereof, and said receptor.

REFERENCES:
patent: 4433059 (1984-02-01), Chang et al.
Klausner, A. Biotechnology, vol. 5, pp. 867-868, Sep. 1987.
Marx, J. Science, vol. 229, pp. 455-456, Aug. 1985.
Tijssen, P., In Practice and Theory of Enzyme Immunoassays pub. Elsevier, Amsterdam, 1985, vol. 15, Chapter 14, pp. 330-336.

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