Process for the detection of nucleic acids

Details Process for the detection of nucleic acids Process for the detection of nucleic acids

1995-01-11

1997-12-30

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, C12Q 168, C07H 2104

Patent

active

057028885

ABSTRACT:
For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound vie a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.

REFERENCES:
patent: 5198537 (1993-03-01), Huber et al.
patent: 5344757 (1994-09-01), Holtke et al.
Langer et al. (1981), Proc. Natl. Acad. Sci USA 78(11): 6633-6637.
Lewis et al. (1987). J. Clin. Pathol. 40: 163-166.
Forster et al. (1985), Nucl. Acids Res. 13 (3): 745-761.
Saiki et al. (1985), Science 230: 1350-1354.

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