Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-12-05
1997-05-20
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 792, 435 795, 435971, 435975, 436518, 436524, 436525, 436527, 436529, G01N 3353
Patent
active
056311362
ABSTRACT:
The present invention relates to a process for determining complexes of .alpha.-1-antichymotrypsin and cathepsin G in a sample comprising adsorbing the cathepsin G portion of the complex to a solid phase coated with non-specific binding protein or gelatin, and detecting the .alpha.-1 portion of the complex with a detectably labelled anti-.alpha.-1-antichymotrypsin antibody. A diagnostic kit comprising the solid phase coated with non-specific binding protein or gelatin and the labelled anti-.alpha.-1-antichymotrypsin antibody is also provided.
REFERENCES:
patent: 4849353 (1989-07-01), Harpel
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"Analysis of the Plasma Elimination Kinetics and Conformational Stabilities of Native, Proteinase-Complexed, and Reactive Site Cleaved Serpins: Comparison of .alpha..sub.1 -Proteinase Inhibitor, .alpha..sub.1 -Antichymotrypsin, . . . Angiotensinogen, and Ovalbumin", Alan E. Mast et. al., Biochemistry, 30(6):1723-1730 (1991).
Havemann Klaus
Heidtmann Hans-Heinrich
Behringwerke Aktiengesellschaft
Spiegel Carol A.
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