Process for the detection of antibodies

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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G01N 3354

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active

043605923

ABSTRACT:
Novel enzyme conjugates useful in immunoassay methods are prepared with the use of a novel coupling reagent of N-succinimidyl (4-iodoacetyl) aminobenzoate by reacting the coupling reagent, firstly, with an amino-containing macromolecule, and, thereafter, with a sulfhydryl-containing enzyme, the enzyme conjugate prepared in a high yield and of high specificity.

REFERENCES:
patent: 3541084 (1970-11-01), Hagitani et al.
patent: 4220722 (1980-09-01), Rowley et al.
Higgins et al., Chem. Abstracts 89: 142590d, (1978).
Santi et al., Chem Abstracts 83: 175111c, (1975).
Kitagawa et al., Journal of Biochemistry 79, 233-236, (1976).

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