Process for the decontamination of cell cultures containing myco

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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514 45, 536 278, C12N5/06;5/08

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active

059027401

ABSTRACT:
The invention relates to a process for the decontamination of cell cultures containing mycoplasmas. In this process the cell cultures infected by mycoplasmas undergo an antibiotic treatment in a first step, and cells still infected are then eliminated by 6-methylpurine deoxyriboside solution in a second step.

REFERENCES:
patent: 4387161 (1983-06-01), McGarrity et al.
patent: 4895803 (1990-01-01), Hubner et al.
Ishiguro, K. et al., "Depletion of Mycoplasma from Infected Cell Lines By Limiting Dilution in 6-methylpurine deoxyriboside", Journal of Immunology, vol. 108, pp. 39-43, (1988).
L.B. Robinson et al., Science 124:1147 (1956).
G.J. McGarrity and D.A. Carson, Exp. Cell Res. 139:199-205 (1982).
R.J. Hay et al., Nature 339:487-8 (1989).
Hay et al. Nature 339:487-488, 1989.
Robinson et al. Science 124: 1147, 1956.
Schmidt et al. Exp. Cell Res. 152: 565-570, 1984.
McGarrity et al. Exp. Cell Res. 139:199-205, 1982.

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