Process for the deacylation of cyclic lipopeptides

Details Process for the deacylation of cyclic lipopeptides Process for the deacylation of cyclic lipopeptides

1998-09-03

2001-03-27

Marx, Irene (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S253500, C435S068100

Reexamination Certificate

active

06207434

ABSTRACT:

TECHNICAL FIELD
The present invention relates to enzyme technology.
The present invention relates to a novel acylase capable of deacylating the acyl side chain of a cyclic lipopeptide compound and to a deacylation method using the same.
More particularly, the invention relates to a novel acylase adapted to deacylate the acyl side chain of Substance FR901379 (described in Japanese Kokai Tokkyo Koho H3-184921), which is produced by the microorganism Coleophoma sp. F-11899 (FERM BP-2635), or an analog of Substance FR901379 and to a deacylation method using the same.
BACKGROUND ART
There has been a standing need for an acylase capable of deacylating the acyl side chain of a cyclic lipopeptide compound, specifically said Substance FR901379 or analog, with good efficiency.
DISCLOSURE OF THE INVENTION
The inventors of the present invention did an extensive research in search of a novel acylase capable of deacylating the acyl side chain of a cyclic lipopeptide represented by Substance FR901379 or its analogs such as Echinocandin B and Aculeacin A. As a result, they discovered an acylase produced by
Streptomyces anulatus
and succeeded in effective achievement of the objective deacylation.
The above novel cyclic lipopeptide acylase and the deacylation method using the same are now described with reference to their salient features.
First, the cyclic lipopeptide acylase-producing microorganism is described.
The novel cyclic lipopeptide acylase-producing microorganism includes but is not limited to
Streptomyces anulatus
No. 4811,
Streptomyces anulatus
No. 8703, and Streptomyces sp. 6907, all of which belong to the genus Streptomyces.
The characteristics of those strains are now described.
The novel cyclic lipopeptide acylase-producing strain named
Streptomyces anulatus
No. 4811 (herein after referred to briefly as Strain No. 4811) was isolated for the first time from a soil sample collected in Fukushima Prefecture. The bacteriological characteristics of this Strain No. 4811 are now described.


REFERENCES:
patent: 4396543 (1983-08-01), Debono
patent: 5573936 (1996-11-01), Kreuzman
Boeck et al., J. Antibiotics, 1988, vol. 41, No. 8, pp. 1085-1092.

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