Process for the continuous production of fructose-1,6-di-phospha

Details Process for the continuous production of fructose-1,6-di-phospha Process for the continuous production of fructose-1,6-di-phospha

1987-07-15

1990-04-24

Richman, Barry S.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435182, C12P 1902, C12N 1104

Patent

active

049200496

ABSTRACT:
The process provides the enzymatic phosphorylation of glucose to FDP by means of yeast immobilized by glutaraldehyde and the separation of FDP by ultrafiltration. A suspension of glutaraldehyde-treated yeast is fed to a fermenter, with addition of a nutrient mixture consisting of dextrose (1M), sodium phosphate (0.5 M), magnesium chloride (10 mM) and phosphoric acid until pH 6.5. The mixture is re-circulated through a hollow fiber ultrafiltration unit to separate the FDP that forms during the phosphorylation reaction.

REFERENCES:
patent: 4355103 (1982-10-01), Boguslawski et al.
patent: 4599311 (1986-07-01), Kawasaki
patent: 4666852 (1987-05-01), Cork
patent: 4683198 (1987-07-01), Ishikawa et al.
patent: 4767707 (1988-08-01), Levesque et al.

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