Process for the continuous enzymatic change of water soluble .al

Details Process for the continuous enzymatic change of water soluble .al Process for the continuous enzymatic change of water soluble .al

1980-07-25

1982-04-20

Bernstein, Hiram

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435180, 435288, C12P 742

Patent

active

043260314

ABSTRACT:
Water soluble .alpha.-ketocarboxylic acids are continuously converted in a membrane reactor into the corresponding .alpha.-hydrocarboxylic acids. The conversion takes place in the presence of a substrate specific dehydrogenase and of a nicotinamide-adenine-dinucleotide (NAD.sup.+ /NADH) enlarged in molecular weight through linkage to a water soluble polymer as coenzyme. Simultaneously NADH is regenerated continuously from NAD.sup.+ in presence of a formate dehydrogenase and from formate ion. The membrane must have a mean pore diameter of 1 to 3 nm. As coenzyme there is employed 0.1 to 10 mmol/l of NAD.sup.+ /NADH present bound to a water soluble polymer having an average molecular weight between 500 and 50,000. There is continuously supplied to the reactor a substrate stream which contains 50 to 100% of the maximum amount soluble, but not over 2,000 mmol/l, of the reacting .alpha.-ketocarboxylic acid in the form of a water soluble salt and 100 to 6,000 mmol/l of a formate. There is maintained over the membrane a differential pressure of 0.1 to 15 bar. There is continuously drawn off behind the membrane a filtrate stream containing the .alpha.-hydroxy acid formed.

REFERENCES:
patent: 4229529 (1980-10-01), Michal et al.
patent: 4266026 (1981-05-01), Breslav
Immobilized Enzymes, 1978, Wiley & Sons, N. Y., Chibata, Editor, pp. 80, 81.

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