Process for the cleavage of peptides and proteins at the methion

Details Process for the cleavage of peptides and proteins at the methion Process for the cleavage of peptides and proteins at the methion

1985-11-07

1987-02-17

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Chemical modification or the reaction product thereof, e.g.,...

435176, 435177, 435207, 530345, 530812, C07K 1700, C12N 999, C12N 1102

Patent

active

046440577

ABSTRACT:
The invention relates to a process for the cleavage of peptides and proteins at the methionyl bond using cyanogen chloride.

REFERENCES:
patent: 3645852 (1972-02-01), Axen et al.
patent: 3876501 (1975-04-01), Hanushewsky
patent: 3919049 (1975-11-01), Kiuchi et al.
patent: 3935072 (1976-01-01), Chibata et al.
patent: 4496689 (1985-01-01), Mitra
Biochem. J. 48, 271-276 (1950), Aldridge.
Methods in Enzymology, vol. XI, 238-255, (1967), Gross.
J. Biol. Chem. 258(1983), 14354-14358, Fowler et al.
Document A: (Gross et al., "Selective Cleavage of the Methionyl Peptides in Ribonuclease with Cyanogen Bromide," The Journal of the American Chemical Society, vol. 83, pp. 1510-1511 (1961)).
Document B: (Ullmanns Encyklopadie Der Technischen Chemie, 4th Ed., vol. 9, p. 6568).
Document C: (Hoben-Weyl, "1. Qualitativer Nachweis," Methoden Der Organischen Chemie, vol. II, p. 545 (1953)).

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