Process for the biotechnological preparation of poly-d-(-)-3-hyd

Details Process for the biotechnological preparation of poly-d-(-)-3-hyd Process for the biotechnological preparation of poly-d-(-)-3-hyd

1984-11-23

1988-11-22

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435829, C12P 742, C12R 105

Patent

active

047865989

ABSTRACT:
The present invention relates to a process for the biotechnological preparation of poly-D-(-)-3-hydroxybutyric acid (PHB) which comprises continuously culturing a microorganism that is a strain of Alcaligenes latus or a PHB-producing mutant thereof, in two separate successive fermentation stages with unrestricted supply of nutrients. The PHB-containing microorganism population cultured in the first stage with a dissolved oxygen content of 25 to 50% of the saturation value for air is then continuously transferred into a second fermentation stage in which the culture is continued at a dissolved oxygen content of 8-15% of the saturation value of air. The PHB is extracted in the usual manner from the biomass thereby obtained.

REFERENCES:
patent: 4138291 (1979-02-01), Lafferty
Nur, Israel et al., Journal of General Microbiology, vol. 128; 1982; pp. 2937-2943.
Malik et al.; "Nitrogen Fixation by the Hydrogen-Oxidizing Bacterium Alcaligenes latus"; Arch. Microbiol., vol. 129, (1981); pp. 254-256.

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