Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Synthesis of peptides
Reexamination Certificate
2006-05-16
2006-05-16
Wax, Robert A. (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Synthesis of peptides
C435S069100
Reexamination Certificate
active
07045593
ABSTRACT:
A method for producing a protein using a cell-free protein synthesis system comprising a detergent so that the protein can be synthesized without aggregation, is provided. The protein is a protein comprising a hydrophobic region in at least a portion thereof, for example, a membrane protein or its fragment (portion). And the detergent is a mild detergent which would not denature the protein, for example, a nonionic or amphoteric ionic detergent.
REFERENCES:
patent: 5270181 (1993-12-01), McCoy et al.
patent: 2002/0142387 (2002-10-01), Seki et al.
patent: 1 143 009 (2001-10-01), None
patent: 1 176 210 (2002-01-01), None
patent: 1 354 959 (2003-10-01), None
patent: 09-107954 (1997-04-01), None
patent: WO 99/02671 (1999-01-01), None
Noshimura et al., Enhancement of Protein Synthesis in Continuous-Flow, Cell-Free System by Improvement of Membrane Permeation, 1995, Journal of Fermentation and Bioengineering, 80(4), 403-405.
Sigma-Aldrich product catalog page for Polyoxyehtylene 23 lauryl ether (Brij 35).
Bochkareva et al., “Chaperonin-promoted Post-translational membrane insertion of a multispanning membrane protein lactose permease” The Journal of Biological Chemistry, 1996, vol. 271, No. 36, pp. 22256-22261.
Wheatley et al., “Glycosylation of G-protein-coupled receptors for hormones central to normal reproductive functioning: its occurence and role”, Human Reproduction Update, 1999. vol. 5, No. 4, pp. 356-364.
Norihiro Nishimura et al.: Journal of Fermentation and Bioengineering, (1995), vol. 80, No. 4, pp. 403 to 405.
Takanori Kigawa et al.: FEBS Letters, (1999), vol. 442, pp. 15 to 19.
P.J. Booth et al.: Biochemical Society Transactions, (2000), vol. 2, part 3, p. A50.
E. Bochkareva et al., the Journal of Biological Chemistry, vol. 271, No. 36, Sep. 1996, pp. 22256-22261.
Ishihara Goushi
Kigawa Takanori
Shirouzu Mikako
Tajima Kaori
Yabuki Takashi
Mayer Suzanne M.
Riken
Wax Robert A.
LandOfFree
Process for synthesizing protein using cell-free protein... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for synthesizing protein using cell-free protein..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for synthesizing protein using cell-free protein... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3553501