Process for synthesizing oligonucleotides with random codons

Details Process for synthesizing oligonucleotides with random codons Process for synthesizing oligonucleotides with random codons

1992-12-14

1993-11-23

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2531, 536 2533, 536 2534, C07H 2100, C07H 2104

Patent

active

052645630

ABSTRACT:
The invention provides a method of synthesizing oligonucleotides having random tuplets using individual monomers. The steps consist of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, the coupling is performed in separate reaction vessels; (2) mixing the supports from the reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2). Additionally, the oligonucleotides can be cleaved from the supports.

REFERENCES:
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patent: 4500707 (1985-02-01), Caruthers et al.
The Biochemistry of Nucleic Acids; 10th Edition (1986) Adams et al. pp. 11-12.
Fed. Proceedings vol. 42, No. 7 (1983) p. 2264 Blake et al.
Cwirla et al. Proc. Natl. Acad: Sci. vol. 87, pp. 6378-6382, 1990.
Devlin et al. Science, vol. 249, pp. 404-406, 1990.
Scott et al. Science, vol. 249, pp. 386-390, 1990.

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