Process for synthesis of peptides

Details Process for synthesis of peptides Process for synthesis of peptides

1993-03-22

2000-03-21

Horlick, Kenneth R.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Synthesis of peptides

530333, C07K 104

Patent

active

06040423&

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND OF THE INVENTION

1. Field of the Invention
The invention relates to methods and apparatus for the synthesis of peptides and oligonucleotides.
2. Brief Description of Related Art
Proteins play a decisive role in practically all biological processes such as enzymatic catalysis, transport and storage, coordinated movement, mechanical supporting function, immune protection, conduction of nerve stimuli and control of growth and differentiation. Proteins are made up of one of more linear polypeptide chains. Their multifarious functions are achieved through three-dimensional folding and the association of these chains. The folding and 3D structure is determined by the primary amino acid sequence of the chains. The multiplicity of protein structures in nature is produced by combining twenty amino acid components. Short partial sequences (oligopeptides) can to a certain extent imitate the local structure and function of a sequence in the total protein formation. Oligopeptides in turn can be prepared by chemical syntheses. Synthetic oligopeptides are thus an important aid in the analysis of the structure and function of proteins.
Because of the size and complexity of proteins, very many peptides are used in systematic studies. For example, localisation of a functional region within a relatively long protein chain using overlapping peptides (cf. M. Z. Atassi, Eur. J. Biol. 145, 1-20 (1984); H. M. Geysen, R. H. Meloen and S. J. Barteling, Proc. Natl. Acad. Sci. U.S.A. 81, 3998 (1984). Also, deciphering the amino acid residues which are essential for function by means of substitution analysis (cf. R. A. Houghton, Proc. Natl. Acad. Sci. U.S.A. 82, 5131 (1985); H. M. Geysen, R. H. Meloen, and S. J. Barteling, Proc. Natl. Acad. Sci. U.S.A. 81, 3998 (1984).
Biological test reactions such as enzymatic reaction and antibody binding are very sensitive, so that even small quantities (ng to .mu.g) of peptide substrates are sufficient. For the rapid and simple implementation of such tests it is advantageous if the peptide substrate is immobilized on a solid support material and can be readily removed from the reaction solution. The reaction with the peptide can then be measured quantitatively either in the remaining reaction solution or by subsequent analysis of the support-bound material.
The extent to which such systematic investigations can be carried out depends essentially on the speed of the peptide synthesis and on the technical and the chemical characteristics of the support. Rapid peptide syntheses can be carried out according to the principle developed by Merrifield of step-wise synthesis on a solid phase (R. B. Merrifield, J. Amer. Chem. Soc. 85, 2149 (1963)).
A process will be described below by which very many (several hundred) immobilized oligopeptides can advantageously be prepared per working day in quantities adequate for test purposes and on a suitable support material or flat material.


SUMMARY OF THE INVENTION

For this purpose, a process is provided according to the invention for the rapid synthesis of support-bound or free peptides, starting from one or more functionalized supports and synthesizing the required peptides step by step corresponding to the given amino acid sequences according to a suitable synthesis procedure, characterized in that or the required dipeptide or oligopeptide, in the form of their activated derivatives, are transferred to the planar support in the shape of spots or lines wetting and support using a suitable cleaving solution.
According to a special embodiment, several to very many different peptides are synthesized simultaneously alongside each other on a flat material on spot-shaped or linear reaction areas which are not in contact with each other.
According to a further special embodiment, the flat material employed is a porous or non-porous film, membrane, or a paper of this nature, which possess a chemically reactive functionality which is suitable for coupling amino acids or peptides.


BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a representation of the primary

REFERENCES:
patent: 4689405 (1987-08-01), Frank et al.
patent: 4873126 (1989-10-01), Bloomster et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5429807 (1995-07-01), Matson et al.
patent: 5436327 (1995-07-01), Southern et al.
Eichler et al., Collect. Czech Chem. Comm. 54, 1746-1752 (1989).
Petmitr et al., Bioch. Biophys. Res. Comm. 162 (2), 846-851 (1989).
Geysen et al., PNAS 81, 3998-4002 (1984).
Fodor et al., Science 251, 767-773 (1991).
Frank, Tetrahedron 48(42), 9217-9232 (1992).
Lebl et al., Peptides 1990, 153-155 (1991).
Eichler et al., Peptide Res. 4(5), 296-307 (1991).

Affiliated with

Also associated with

Rating

Process for synthesis of peptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for synthesis of peptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for synthesis of peptides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-731347