Plant husbandry – Process
Patent
1982-12-02
1984-10-23
Bagwill, Robert E.
Plant husbandry
Process
A01G 100
Patent
active
044780001
ABSTRACT:
The present invention embodies a process for mass propagation of plant tissue preferably of a Simmondsia chinensis, commonly known as Jojoba, and plant tissue having similar characteristics thereto. The process is directed to asexual reproduction of plantlets from tissue of immature and mature plants from a single piece of donor tissue and involves performing the steps thereon of: sterilization; chemical pretreatment with a cytokinin to increase the likelihood of tissue survival; washing again in sterile water; placing and retaining for a specified period the prepared tissue on first and second culture media to support and stimulate tissue and shoot development; separating the shoots by surgical division and transferring to a shoot multiplication medium for further development for a desired period; then transferring to a rooting medium for development into plantlets which are then transferred to a soil mix. The process of the present invention including the steps of the chemical pretreatment soak in a cytokinin of preferred concentrations; the use of certain chemicals in the culture media are complementary to the pretreatment soak and provide for an increase in plantlet survival and multiplication over former processes.
REFERENCES:
Article-JoJoba Reports, "Micropropagation for Jojoba Improvement Programs," by Zachary S. Wochok & Carolyn J. Sluis, No. 26, Mar. 1979, pp. 1-5.
Birnbaum, Elliott, Dept. of Natural Products, Institute for Applied Research, Ben-Gurion University of the Negev, Beer-Sheva, Israel, 1978, Simmondsia chinensis: Studies by Tissue Culture, pp. 243-252 in A. W. Alfermann and E. Reinhard, eds., Production of Natural Compounds by Cell Culture Methods.
Mandani, A., C. W. Lee and L. Hogan, 1978, In vitro propagation of Simmondsia chinensis Via Shoot Tip Cultures, HortScience 13(3):35.
Rost, T. L. et al., The Production of Jojoba Wax in Tissue Culture, p. 14 in Abstracts, Third International Conference on Jojoba, Riverside, California, Sep. 13-16, 1978, 18 p.
Hogan, LeMoyne, Chi Won Lee, David A. Palzkill and William R. Feldman, "Recent Progress in the Propagation of Jojoba by Stem Cuttings", pp. 1-4 in a Report of a Third International Conference on Jojoba, Riverside, California, Sep. 13-16, 1978.
Tautvydas, Kestutis J. "Organogensis in Tissue Culture of Jojoba (Simmondsia chinensis)," pp. 25-38 in a Report of a Third International Conference on Jojoba, 1978.
Rost. T. L. and Maud A. W. Hinchee, Preliminary Report of the Production of Callus, Organogensis and Regeneration of Jojoba (Simmondsia Chinensis, Link, Schneid. ) in Tissue Culture, pp. 299-305 in Journal of Horticultural Science (1980) 55 (3).
Sluis Carolyn J.
Wochok Zachary S.
Bagwill Robert E.
Plant Resources Institute
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