Process for stabilizing plasmids by deletion of DNA

Details Process for stabilizing plasmids by deletion of DNA Process for stabilizing plasmids by deletion of DNA

1982-02-25

1983-08-30

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 70, 435 68, 435253, 435317, C12N 1500, C12P 2102, C12P 2100, C12N 120, C12N 100

Patent

active

044017618

ABSTRACT:
Novel chemical compounds, recombinant plasmids pUC1026 and pUC1027, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6. These plasmids are produced by a novel process which can be used to stabilize unstable potential plasmid vectors. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin. The stabilization process disclosed herein can be used to make other stable plasmids.

REFERENCES:
Timmis; in Plasmids of Medical, Environmental and Commercial Importance, Timmis et al., (ed.), Elsevier/North Holland, 1979, pp. 13-22.
Thomas et al., in Plasmids of Medical, Environmental and Commercial Importance, Timmis et al., (ed.), Elsevier/North Holland, 1979, pp. 375-385.
Thompson, C. J., et al., "DNA Cloning in Streptomyces; Resistance Genes from Antibiotic-Producing Species." Nature 286, 525-527.
Suarez, J. E. et al., "DNA Cloning in Streptomyces . . ." Nature 286, 527-529.
Bibb, M. et al., "A DNA Cloning System for Interspecies Gene Transfer in Antibiotic-Producing Streptomyces." Nature 284, 526-531.

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