Process for stabilizing 1-methylhydantoinase, use and method of

Details Process for stabilizing 1-methylhydantoinase, use and method of Process for stabilizing 1-methylhydantoinase, use and method of

1992-01-16

1994-12-20

Czaja, Donald E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435 18, 435 29, 435227, C12N 996, C12N 978

Patent

active

053745469

ABSTRACT:
The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.

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