Process for specimen handling for analysis of nucleic acids

Details Process for specimen handling for analysis of nucleic acids Process for specimen handling for analysis of nucleic acids

1993-10-12

1995-07-25

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435287, 435299, 436177, 436178, 436515, 436516, 422 56, 422 58, 422 61, 422 69, 422101, 422102, 422104, 2041826, 2041828, 2041829, 204299R, 935 76, 935 77, C12Q 168, C12P 1934, C25B 100, B01D 6142

Patent

active

054361290

ABSTRACT:
A process for handling a biological specimen for the analysis of nucleic acid sequences wherein the biological specimen is immobilized within a carrier device for controlled temperature conditions and the sequential addition of fluid treatments. The fluid treatments arm selected from lysing end denaturing solutions, wash or rinse solutions, reagents for nucleic acid amplification, electrophoresis and hybridization and labeling and detection reagents. The fluid treatments are unique to each specimen and the detection of target nucleic acid sequences is localized within the carrier device.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis
patent: 5021335 (1991-06-01), Tecott et al.
patent: 5024934 (1991-06-01), Lee
Hurley et al., Biopolymers, vol. 25, pp. 539-554, issued 1986.
Tecott et al., Science, vol. 240, pp. 1661-1664, issued 17 Jun. 1988.
Nucleic Acids Research, Sommer and Tautz, "Minimal homology requirements for PCR", vol. 17, No. 16, 1989, p. 6749.

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