Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol
Patent
1983-02-22
1985-10-01
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving cholesterol
435 11, 435 19, 436 71, C12Q 160, C12Q 144
Patent
active
045446300
ABSTRACT:
The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0.
The present invention also provides a reagent for carrying out this process.
REFERENCES:
patent: 4226713 (1980-10-01), Goldberg
Bartl Knut
Roder Albert
Wehmeyer Gunter
Ziegenhorn Joachim
Boehringer Mannheim GmbH
Marantz Sidney
Wieder Stephen C.
LandOfFree
Process for specific determination of cholesterol in the presenc does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for specific determination of cholesterol in the presenc, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for specific determination of cholesterol in the presenc will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1442546