Plastic and nonmetallic article shaping or treating: processes – Forming continuous or indefinite length work – Shaping by extrusion
Patent
1985-07-31
1989-07-18
Hill, Jr., Robert J.
Plastic and nonmetallic article shaping or treating: processes
Forming continuous or indefinite length work
Shaping by extrusion
435 6, 436 94, 935 77, G01N 3000, G01N 3350
Patent
active
048490776
ABSTRACT:
A process for solid phase sequencing of nucleic acid fragments is disclosed. The object of the invention is to provide a sequencing process which enables the simultaneous sequencing of large amounts of long and short nucleic acid fragments and which is optionally automated. Thus, a solid support combining mechanical stability, anion exchange characteristics, and chemical elution of nucleic acids off the support is used. Immobilized nucleic acid fragments are chemically modified and subsequently the nucleic acid backbone is cleaved and simultaneously or subsequently said fragments are eluted by chemical means. The present invention is applied to molecular biology and gene technology.
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patent: 4542102 (1985-09-01), Dattagupta et al.
patent: 4588682 (1986-05-01), Groet et al.
patent: 4713326 (1987-12-01), Dattagupta et al.
Chuvpilo et al., Khim., vol. 9, pp. 1634-1637 (1983).
Sequencing End-Labeled DNA with Base-Speific Chemical Cleavages, Author: By Allan M. Maxam and Walter Gilbert, pp. 499-561, Methods in Enzymol, 65 (1980).
Graatschus Monika
Hunger Hans-Dieter
Kagelmaker Horst
Rosenthal Andre
Akademie der Wissenschaften der DDR
Hill Jr. Robert J.
Striker Michael J.
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