Process for simultaneously detecting multiple antigens using dua

Details Process for simultaneously detecting multiple antigens using dua Process for simultaneously detecting multiple antigens using dua

1984-04-04

1987-09-01

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 21, 4351722, 43524027, 435948, 435810, 436531, 436533, 436548, 436808, 436813, 436815, 436819, 935 89, 935110, G01N 3353, G01N 33545, C12Q 142, C12N 1500

Patent

active

046908900

ABSTRACT:
At least two antigens in a sample may be detected using an immunometric dual sandwich assay containing an effective amount of at least one monoclonal antibody against each antigen, which antibodies are separately conjugated with the same or different signal moieties as labels, and an effective amount of at least one unlabeled monoclonal antibody against each antigen which unlabeled antibodies are immobilized on a single support. Preferably the antibodies are all products of different cell lines and the antigens are prostatic acid phosphatase and prostate antigen.

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J. Ljunggren et al., Acta Endocrinologica, 81, 487-494 (1976).
Hybritech Tandem (TM) Pap Kit brochure (1981) entitled, "Immunoradiometric Assay for the Quantitative Determination of Prostatic Acid Phosphatase".
Abbott Laboratories Trade brochure (10/13/81) on trade name product Abbott PAP-EIA.
Vihko et al., Clinical Chemistry, vol. 27, p. 1744 (1981).
Larsson, J. Histochem. Cytochem., 1981, vol. 29, pp. 408-410.
Dyminski et al., 67th Annual Meeting of the Federation of American Societies for Experimental Biology, Chicago, IL, U.S.A., Apr. 10-15, 1983, Fed. Proc. 42 (3) 1983, Abstract 873.

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