Process for serially culturing keratinocytes

Details Process for serially culturing keratinocytes Process for serially culturing keratinocytes

1975-11-14

1977-04-05

Rosen, Sam

Textiles: fiber preparation

C12K 900

Patent

active

040160360

ABSTRACT:
A method is disclosed for serially culturing keratinocytes such as human epidermal cells and teratomal keratinocytes. These can be serially grown in culture to produce colonies and eventually stratified squamous epithelium by including in the culture fibroblast cells or medium harvested from fibroblast cultures. When fibroblast cells are used, they are treated to prevent their multiplication, and they are also introduced into the cultures at a controlled density.

REFERENCES:
willmer--Cells and Tissues in Culture vol. 2 (1965) pp. 656 and 657.

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