Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1995-10-06
1999-02-16
Scheiner, Toni R.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530385, 530829, 435183, 4353171, 424647, 424529, 426647, G01N 3349, A23J 106, A23J 312, A61K 3514
Patent
active
058722270
DESCRIPTION:
BRIEF SUMMARY
This application is a 371 of PCT/SE94/00314 filed Apr. 8, 1994.
DESCRIPTION
1. Technical Field
The present invention is related to a process for separation of components from red blood cells.
The object of the invention is to facilitate separation of cell membranes from a water soluble fraction. The invention thereby enables effective manufacture of raw material for preparation of membrane-bound lipids from red blood cells. A further object of the invention is to facilitate purification of components from a water soluble fraction of red blood cells, by reducing the lipid content of such water-soluble preparation. A further object is to facilitate preparation of glycolipids or phospholipids in a lipid extract of said membranes.
2. Background of the Invention
In separation of cell membranes and a water soluble fraction from red blood cells for analytical and preparative purposes, usually a centrifuge method is employed. An effective separation of native cell membranes from a water soluble fraction requires a great gravitational force. High gravitational forces and an acceptable product flow could not be combined on processing of red blood cells on an industrial scale. This has lead to low yields of cell membranes.
With the purpose of increasing the yield of blood fractions on centrifugal preparation, supplementary techniques have been developed, for example a system of blood bags in combination with pressure cushions (EP-A-026417) and synthetic gels which separate cells from a water soluble fraction (EP-A1-520185). These techniques are effective on processing of small volumes of blood, but they are not suitable for industrial production.
Further, a filtration technique is employed for enrichment of whole red blood cells or specific fractions (Japanese Patent Application No. 61,181,963, U.S. Pat. No. 4,946,603). Cell membranes, however, disturb the filter, thus that the flow is limited, and thereby the capacity of the process is reduced.
It is previously known that aggregation or agglutination of blood cells can be achieved by cross-linking the negative surface charges of the blood cell with amine polymers such as polylysine (EP-A-438192) and chitosan (Senstad C. and Mattiasson B. Biotechnology and Bioengineering 34(3), 387-393, 1989). This type of aggregation can facilitate separation of blood cells from a water soluble fraction. Production of polylysine is resource-demanding, which limits the utility as means for aggregation of cell membranes on an industrial scale. Chitosan aggregates cell membranes, but simultaneously increases the viscosity of the solution. The increased viscosity counteracts the sedimentation of cell membranes induced by the aggregation. The cross linking amine polymers adhere to components of the surface of the cell membrane, e.g. sialic acid on glycolipids and glycoproteins. On preparation of native forms of e.g. biologically active glycolipids, an extra separation step may be required, which removes bound amine polymer.
GB-A-1430 217 describes a process for the preparation of an infusible storage-stable haemoglobin solution by haemolysing an erythrocyte-containing starting material with .beta.-propiolactone, washing out the .beta.-propiolactone, and separating the stroma from the hemoglobin solution by binding the stroma to a cation exchange material at pH 5.0 to 5.5.
SU Inventor's Certificate 940 066 describes a process for production of haemoglobin and stroma for medical use. Erythrocytes in buffer solution are haemolysed in a buffer solution, in particular a sodium phosphate buffer of pH 6.1-6.5, followed by separation of the free haemoglobin from cell stroma by binding of hemoglobin to a cation exchange material while the stroma remains in the surrounding medium.
DESCRIPTION OF THE INVENTION
The present invention provides a process for recovering one or more components from red blood cells, whereby a composition comprising red blood cells is made subject to a separation step where lipid-containing cell membranes and water soluble components are separated, and whereby the li
REFERENCES:
patent: 4376727 (1983-03-01), Sato
patent: 4610814 (1986-09-01), Dede
Senstad, C. and Mattiasson, B.; Biotechnology and Bioengineering 34, pp. 387-393, 1989; Purification of Wheat Germ Agglutinin Using Affinity Flocculation with Chitosan and a Subsequent Centrifugation or Flotation Step.
Dialog Information Services, File 73, Embase, Dialog accession No. 1464221, Embase accession No. 79234798; Chuillon, J. et al.; J. Fr. Biophys. Med. Nucl. (France), 1979, 3/3 (121-126).
Patent Abstracts of Japan, "Hepatitis-Free Haemoglobin Solutions", vol. 85, No. 25, abstract of JP, A, 85-25411, 1985.
Sigma catalog 1992 pp. 511-513 and 792-799.
Erlansson Karin
Jungvid Hans
Mattiasson Bo
Nilsson Goran
Olin Thomas
Gramineer AB
Johnson Nancy A.
Scheiner Toni R.
LandOfFree
Process for separation of components from red blood cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for separation of components from red blood cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for separation of components from red blood cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2063381