Process for separation and isolation of AHF and fibronectin from

Details Process for separation and isolation of AHF and fibronectin from Process for separation and isolation of AHF and fibronectin from

1979-06-19

1980-07-01

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

424101, A23J 106, A61K 3516, C07G 700

Patent

active

042105803

ABSTRACT:
A process for the separation and isolation of AHF and fibronectin from blood plasma by adding a sulfated mucopolysaccharide to blood plasma to a concentration of 0.15-0.25 mg/ml of plasma, cooling the plasma to 0.degree. C.-15.degree. C. centrifuging the plasma to isolate a precipitate which forms and then removing the AHF-rich supernatant liquid from the precipitate.
The precipitate is washed in a slightly basic solution of dilute salts and then dissolved in a buffered solution of chaotrophic salts of the Hofmeister series having an ionic strength from 0.01-1. Fibronectin is then separated, by chromotographic means, from the dissolved precipitate, which contains fibronectin and fibrinogen.
In a preferred embodiment, 0.20 mg of sodium heparin per milliliter of human plasma is employed with the heparinized plasma solution cooled for 3 hours at a temperature of 2.degree. C.-4.degree. C.

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Proc. 7th Congress European Soc. Haematology, London, 1959, Part II, pp. 587-593, (1960), Blomback et al.
J. Biol. Chem. (1973), 248 (24), pp. 8429-8433, Sawyer et al.

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