Process for separating complex

Details Process for separating complex Process for separating complex

1994-12-12

1996-11-05

Saunders, David

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

210656, 210660, 210668, 435 71, 435 74, 436538, 436824, 530412, 530416, 530417, G01N 33538, G01N 33573

Patent

active

055717292

ABSTRACT:
This invention is intended to provide a process by which a complex formed by the interaction between one or more analytes to be measured and affinity substance can be more effectively separated from substances existing together therewith which tend to affect the detection of the complex, for example, free affinity substance, by using high pressure liquid chromatography (HPLC); and a process for measuring a trace component by utilizing said separating process.
This invention is characterized in that a substance which has been modified with a substance capable of changing properties of the complex (a separation-improving substance) and has affinity for the complex is attached to the complex. Because of this characteristic, the invention is effective in that the position of elution of said complex in the HPLC can be freely controlled. Furthermore, it is markedly effective in that the process for measuring a trace component by utilizing the separating process of this invention makes it possible to carry out the measurement with higher precision as compared with conventional measuring processes using HPLC.

REFERENCES:
patent: 5340474 (1994-08-01), Kauvar
patent: 5352616 (1994-10-01), Sundrehagan
Pierce 1988 Handbook & General Catalog, pp. 128-131 (Rockford, Illinois, 1988).
Fausnaugh et al, "Comparison of Hydrophobic-Interaction and Reversed-Phase Chromatography of Proteins", J. Chromat., 317:141-155 (1984).
Carty et al, "Use of High-Performance Liquid Chromatography for the Purification of Antibodies and Antibody Conjugates and the Study of Antibody-Antigen Interactions", J. Chromat., 442: 279-288 (1988).
Anal. Chem., 1993, 65, 613-616, "Liquid-Phase Binding Assay of Human Chorionic Gonadotropin Using High-Performance Liquid Chromatography", Kenji Nakamura et al.

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