Process for selective peptide bond cleavage using sulfoxides and

Details Process for selective peptide bond cleavage using sulfoxides and Process for selective peptide bond cleavage using sulfoxides and

1987-04-22

1988-05-17

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Chemical aftertreatment, e.g., acylation, methylation, etc.

530303, 530308, 530329, 530344, 530397, 530399, 530406, 530408, 530410, C07K 1700

Patent

active

047451785

ABSTRACT:
This application describes a process for selectively cleaving a peptide or protein at one or more of its tryptophan residues. The process comprises treating in trifluoroacetic acid said peptide or protein at a concentration of from about 0.05 mM to about 50 mM with an organic sulfoxide at a concentration of from about 0.01M to about 1M, chloride ion at a concentration of from about 0.01M to about 2M, and water at a concentration not in excess of about 10M.

REFERENCES:
patent: 4644057 (1987-02-01), Bicker et al.
Shechter, Y., Patchornik, A., and Burstein, Y., Biochem. 15, 5071-5075 (1976).
Lischwe, M. A., and Sung, M. T., J. Biol. Chem. 252, 4976-4980 (1977).
Savige, W. E., and Fontana, A., Methods Enzymol, 47, 459-469 (1977).
Ozols, J., and Gerard, C., J. Biol. Chem. 252, 5986-5989 (1977).
Savige, W. E., and Fontana, A., Methods Enzymol. 47, 442-453 (1977).
Fontana, A., Savige, W. E., and Zambonin, M., in "Methods in Peptide and Protein Sequence Analysis", (Birr, C., Ed.) Elsevier/North-Holland Biomedical Press, 309-322 (1980).
Fontana, A., in "Biochemical and Medical Aspects of Tryptophan Metabolism", (Hayaishi, O., Ishimura, Y., and Kido, R., Editors) Elsevier/North-Holland Biomedical Press, 59-72 (1980).
Huang, H. V., Bond, M. W., Hunkapillar, M. W., and Hood, L. E., Methods Enzymol. 91, 318-324 (1983).
Fontana, A., Methods Enzymol. 25, 419-423 (1972).

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