Process for secretory production of a calcium-binding protein

Details Process for secretory production of a calcium-binding protein Process for secretory production of a calcium-binding protein

1992-07-13

1994-02-22

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 4353201, C12N 1562, C12N 1570

Patent

active

052886239

ABSTRACT:
A process for secretory production of a calcium-binding protein very useful for producing heterogenic proteins in Escherichia coli according to gene recombinant technique is provided, which process comprises using Escherichia coli and using a secretory expression system with the promoter of lipoprotein and the gene of signal peptide of outer membrane protein A, according to recombinant DNA technique.

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The EMBO J. vol. 6: 2835-2841, 1987, Mackman et al. Release of a chimeric protein into the medium from Escherichia coli using the -C-terminal secretion . . .
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PNAS 82: 3154-3158, May 1985, Inouye et al. Cloning and sequence analysis of CDNA for the luminescent protein aequorin.
EMBO J, 4: 1075-1080, 1985, Nilsson et al. Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.
EMBO J, 3: 2437-2442, Mar. 1984, Ghrayeb et al. Secretion cloning vectors in Escherichia coli.
J. Biochem. 105: 473-477, Mar. 1989, S. Inouye et al. Over expression and Purification of the Recombinant Ca.sup.2+ Binding Protein, Apoaequorin.
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