Process for screening for malignant tumors by determining the am

Details Process for screening for malignant tumors by determining the am Process for screening for malignant tumors by determining the am

1994-09-13

1997-02-25

Gitomer, Ralph J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435810, 436 64, 436813, C12Q 148, G01N 3348

Patent

active

056058071

ABSTRACT:
A process is provided for screening for a malignant tumor. The process comprises the steps of determining the amount of GlcNAc transferase V activity in a tumor sample by reacting the GlcNAc transferase V in a tumor sample with an acceptor substrate and a sugar nucleotide donor to produce a detectable change, and detecting the change. The acceptor substrate is an oligosaccharide, a glycopeptide or a glycoprotein having the structure GlcNAc .beta.1-2Man .alpha.1-6Man-R.sub.1. R.sub.1 is GlcNAc .beta.1-4GlcNAc with or without fucose or a synthetic linker. The sugar nucleotide donor is uridine diphospho-N-acetylglycosamine. The amount of GlcNAc transferase V activity in the sample is thereby determined. A determination of the malignancy of the tumor is made by comparing the amount of GlcNAc transferase V activity in the sample with an amount of GlcNAc transferase V associated with normal tissues or with known malignant tumors. A diagnostic kit for screening for a malignant tumor is provided. The kit comprises an acceptor substrate and a sugar nucleotide donor to interact with GlcNAc transferase V in a tumor sample to produce a detectable change, and means for detecting the change.

REFERENCES:
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patent: 5173407 (1992-12-01), Uemura
patent: 5206356 (1993-04-01), Pierce
Dennis J., Oncogenes Conferring Metastatic Potential . . . Oncogene (1989) 4 853-860.

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